c trachomatis major outer membrane protein  (Bio-Rad)

Journal: Science Advances

Article Title: Single-cell atlas of cervical organoids uncovers epithelial immune heterogeneity and intercellular cross-talk during Chlamydia infection

doi: 10.1126/sciadv.ady1640

Figure Lengend Snippet: ( A ) Immunofluorescence images of ectocervical (top) and endocervical (bottom) organoids, uninfected (left) or infected (right) for 48 hours with Chlamydia, stained for KRT5 (green), major outer membrane protein (MOMP) (red), KRT8 (gray), and DAPI (blue). ( B ) UMAP projection of single cells from ecto- and endocervical organoids, colored by infection status: uninfected (UI), infected (Inf), and bystander (Bstd). ( C and D ) UMAP showing reclustered ectocervical squamous epithelial population from (B), colored by infection status (C) and subtype identity (D). ( E ) Proportion of UI, Bstd, and Inf cells in each ectocervical squamous subtype. ( F to G ) UMAP showing reclustered endocervical columnar epithelia from (B), colored by infection status (F) and subtype (G). ( H ) Proportion of UI, Bstd, and Inf cells in each endocervical columnar subtype. ( I ) Heatmap of differentially regulated TFs between ecto- and endocervix across infection conditions; color bar depicts the TF activity scores from high (deep pink) to low (blue). ( J ) Violin plot of gene set enrichment scores for the GO term defense response to bacterium across epithelial compartments and infection states; statistical significance assessed by Wilcoxon rank-sum test with Holm-adjusted P values ( ****P ≤ 0.0001). ( K ) The relative expression of IFN-related genes across ecto- and endocervical subclusters; dot size represents the % of cells expressing a particular gene, and the color bar indicates the intensity of scaled mean expression levels ranging from high (red) to low (blue). ( L ) Gene-weighted density UMAP projections showing expression of STAT1 , STAT2 , and IRF9 across epithelial cells in (B). ( M ) Violin plot showing ISG15 expression across ecto- and endocervical organoids in uninfected, bystander, and infected states. ( N ) IHC images showing CDH1 (green), ISG15 (red), MOMP (gray), and DAPI (blue) in ecto- and endocervical organoids, uninfected (left) or infected (right). Yellow arrows mark infected cells; arrowheads indicate ISG15 + bystander cells.

Article Snippet: The following primary antibodies were used for immunofluorescence: mouse anti–acetylated tubulin–Alexa Fluor 647 (1:300, Santa Cruz Biotechnology, sc-23950-AF647), mouse anti–E-cadherin–Alexa Fluor 488 (1:50, BD Biosciences, 560061), mouse anti–E-cadherin (1:50, BD Biosciences, 610181), rabbit anti–KRT5–Alexa Fluor 488 (1:300, Abcam, ab193894), mouse anti-MUC5B (1:200, Abcam, ab77995), rabbit anti-MUC21 (1:200, ProteinAtlas, HPA052028), rabbit anti-KRT8 (1:200, Abcam, ab59400), mouse-anti-KRT6 (1:50, Abcam, ab18586), recombinant rabbit anti-PAX8 (1:200, Abcam, ab239363), goat anti– C. trachomatis major outer membrane protein (1:500, Bio-Rad, 1990-0804), rabbit anti–HLA-DQA1 antibody (EPR7300) (1:200, Abcam, ab128959), rabbit anti-ISG15 polyclonal antibody (1:200, Proteintech,15981-1-AP), and for labeling the DNA, 4′,6-diamidino-2-phenylindole (DAPI, Roche, 10236276001) were used.

Techniques: Immunofluorescence, Infection, Staining, Membrane, Activity Assay, Expressing

Journal: Rheumatology (Oxford, England)

Article Title: A potential role for chlamydial infection in rheumatoid arthritis development

doi: 10.1093/rheumatology/kead682

Figure Lengend Snippet: Prevalence of anti-MOMP IgG antibodies in serum of RA-FDR defined by their preclinical status

Article Snippet: To explore the robustness of our primary exposure, we performed a nested case–control study in a subset of RA-FDRs for a secondary exposure of interest, the serology against C. trachomatis major outer membrane protein (MOMP) IgG (ELISA, Vircell, Granada, Spain, cat. no. G/M1017).

Techniques:

Journal: Scientifica

Article Title: Lycopene Inhibits Propagation of Chlamydia Infection

doi: 10.1155/2017/1478625

Figure Lengend Snippet: Dose-dependent inhibition of C. trachomatis growth in B10.MLM cells at 42 hpi in the presence of oil-formulated lycopene. (a) C. trachomatis infection in B10.MLM cells at 42 h.p.i. (1) growth in the presence of 0.015% olive oil in DMSO; (2) growth in the presence of 0.75 µ g/ml; (3) 1.5 µ g/ml; and (4) 3.0 µ g/ml of oil-formulated lycopene. Scale bar 100 µ m. (b) Quantitative representation of the inclusion numbers of control and lycopene treated cells. IFU/FOV = Average Inclusion Forming Units per Field of View ( n = 20). (c) Infectious yield after treatment with different doses of lycopene.

Article Snippet: Permeabilized cells were stained for direct immunofluorescence (IF) using FITC—conjugated species-specific monoclonal antibody against the major outer-membrane protein of C. trachomatis (Bio-Rad), or FITC—conjugated monoclonal antibody against chlamydial lipopolysaccharide (Nearmedic Plus, RF).

Techniques: Inhibition, Infection

Journal: Scientifica

Article Title: Lycopene Inhibits Propagation of Chlamydia Infection

doi: 10.1155/2017/1478625

Figure Lengend Snippet: Dose-dependent inhibition of C. trachomatis growth in B10.MLM cells at 42 hpi in the presence of microencapsulated lycopene. (a) C. trachomatis infection in B10.MLM cells at 42 h.p.i. (1) growth in the presence of 1.0% cyclodextrin; (2) growth in the presence of 0.125 mg/ml; (3) 0.25 mg/ml; and (4) 0.5 mg/ml of microencapsulated lycopene. Scale bar 100 µ m. (b) Quantitative representation of the inclusion numbers of control and lycopene treated cells. IFU/FOV = Average Inclusion Forming Units per Field of View ( n = 20). (c) Infectious yield after treatment with different doses of lycopene.

Article Snippet: Permeabilized cells were stained for direct immunofluorescence (IF) using FITC—conjugated species-specific monoclonal antibody against the major outer-membrane protein of C. trachomatis (Bio-Rad), or FITC—conjugated monoclonal antibody against chlamydial lipopolysaccharide (Nearmedic Plus, RF).

Techniques: Inhibition, Infection

Journal: Scientifica

Article Title: Lycopene Inhibits Propagation of Chlamydia Infection

doi: 10.1155/2017/1478625

Figure Lengend Snippet: Lycopene treatment disrupts chlamydial developmental cycle in alveolar macrophages B10.MLM. ((a) and (b)) C. trachomatis infection at 42 hpi without lycopene (EB: elementary body and RB: reticulate body); ((c) and (d)) C. trachomatis infection at 42 hpi treated with oil-formulated lycopene (ARB: abnormal reticulate body); ((e) and (f)) C. trachomatis infection at 42 hpi treated with microencapsulated lycopene. Lipid droplets are in close contact to and inside chlamydial inclusions. Arrows indicate lipid droplets. Bar = 0.25 µ m.

Article Snippet: Permeabilized cells were stained for direct immunofluorescence (IF) using FITC—conjugated species-specific monoclonal antibody against the major outer-membrane protein of C. trachomatis (Bio-Rad), or FITC—conjugated monoclonal antibody against chlamydial lipopolysaccharide (Nearmedic Plus, RF).

Techniques: Infection